Mastering Bulk RNA-seq: Plan, Analyse and Interpret

Where / how can I run an RNA-seq pipeline ?

At Monash:

Web-based:

• laxy.io → Degust
• Galaxy Australia

Command line:

• M3 HPC cluster (eg using https://nf-co.re/rnaseq )
• NeCTAR virtual machine (~32 - 64Gb RAM)
• Your laptop .. while not recommended, you may be able to do this for smaller genomes (bacteria, yeast), and larger genomes if using the Salmon pseudo-mapping protocol